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Longer-term impact of hemiparetic stroke on skeletal muscle metabolism - a pilot study Klaer, Jennifer Hintergrund: Die metabolischen Prozesse in situ sind auf muskuloskelettaler Die metabolischen Prozesse in situ sind auf muskuloskelettaler Gewebeebene bislang jedoch nicht untersucht worden.
Telomerase, a cellular reverse transcriptase enzyme that can counteract telomere shortening and, in addition, preserves healthy cell function, constitutes a promising target for research into the pathological effects of stress on cellular aging.
Previous studies that have included measures of telomerase have typically measured telomerase expression or activity under basal resting conditions.
It is, however, challenging to reliably quantify or interpret these data because leukocyte telomerase is typically expressed at very low levels and is dynamic in nature.
In contrast to basal measures, telomerase activity can be quantified in response to an ecologically-valid challenge such as mitogen stimulation in vitro. The potential advantage of this approach is that it may bypass the above-mentioned limitations to provide an indicator of individual differences in the capacity of the telomere biology system to respond to an immunological challenge.
The aim of this study was to validate an in vitro measure of leukocyte maximal telomerase activity capacity mTAC for use in human studies of telomere biology, and to determine its association with measures of stress and stress responsivity. First, the optimal post-stimulation time course to characterize mTAC was established using an in vitro mitogen challenge phytoheamagglutinin PHA supplemented with interleukin IL Next, mTAC was measured in leukocytes and cortisol concentrations were assessed in saliva obtained from 28 healthy young women and men at different times of the day and before and after a standardized laboratory stressor.
In addition, immune cell distributions prior to mitogen stimulation were determined by flow cytometry in a subset of the participants.
Perceived chronic stress also was assessed using the Perceived Stress Scale. The optimal time point to quantify human leukocyte mTAC was 72 hours after mitogen stimulation.
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A significant proportion of the between-subject variability in mTAC was associated with measures of stress and stress responsivity. Based collectively on these findings, it appears that mTAC may represent a potentially useful individual difference measure in stress-related studies of the human telomere biology system.
Der endoprothetische Ersatz des Schultergelenkes hat in den letzten Jahren progredient zugenommen, ebenso die Entwicklung neuer Endoprothesen. Bisher gibt es zu dieser Endoprothese noch keine publizierten Studien. In dieser prospektiven, nicht randomisierten Multicenterstudie wurden im Zeitraum von November — November an neun Zentren in Europa insgesamt Sidus-Endoprothesen bei Patienten implantiert.
Insgesamt wurden 73 Total- und 32 Hemiendoprothesen implantiert.
Patienten mit einer Totalendoprothese schnitten dabei signifikant besser ab als Patienten mit einer Hemiendoprothese. Radiologisch wurden weder Lockerungen noch Migration der Komponenten beobachtet. This makes anaphylaxis relevant for emergency medicine.
The objective of this study was to examine the treatment of anaphylaxis in various structures of emergency care in Germany and to determine possible diagnostic markers for detecting a severe allergic reaction with an unspecific clinical manifestation.Dissertation Munchen dissertation munchen Munich Digital Humanities work group (dhmuc.): Munich Digital Humanities Summer School; Publishing.
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