Determination of copper concentrations in water samples using spectrophotometry and bicinchoninic ac

March Purpose of consultation The Federal-Provincial-Territorial Committee on Drinking Water CDW has assessed the available information on copper with the intent of updating the current drinking water guideline and guideline technical document on copper in drinking water. The purpose of this consultation is to solicit comments on the proposed guideline, on the approach used for its development and on the potential economic costs of implementing it, as well as to determine the availability of additional exposure data. The proposed MAC is considered to be protective of both short-term effects i.

Determination of copper concentrations in water samples using spectrophotometry and bicinchoninic ac

Biochemistry: Practical 2 : Protein Experiment

Advanced Search Abstract The Lowry and Bradford assays are the most commonly used methods of total protein quantification, yet vary in several aspects. To date, no comparisons have been made in skeletal muscle. We compared total protein concentrations of mouse red and white gastrocnemius, reagent stability, protein stability and range of linearity using both assays.

However, Bland—Altman analysis revealed considerable bias Both Lowry reagents and its protein—reagent interactions were less stable over time than the Bradford.

The linear range of concentration was smaller for the Lowry 0.

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We conclude that the Bradford and Lowry measures of total protein concentration in skeletal muscle are not interchangeable.

The Bradford and Lowry assays have various strengths and weaknesses in terms of substance interference and protein size. However, the Bradford provides greater reagent stability, protein—reagent stability and range of linearity, and requires less time to analyse compared to the Lowry assay.

All rights reserved Issue Section:Copper accumulation in gill tissue significantly increased in a time (P = ) and concentration dependent manner during the exposure schwenkreis.comre to 40 μg Cu L − 1 caused a significant rise in gill copper levels of about % at day 3 and % at day 7, stabilized during day 14 and finally reached a maximum increase of about % at day Apr 03,  · Protein samples and standards are processed in the same manner by mixing them with assay reagent and using a spectrophotometer to measure the absorbance.

The responses of the standards are used to plot or calculate a standard group 4 biochem. After acidification to 1% with concentrated nitric acid, they were stored in polyethylene bottles.

For the preconcentration procedure, pH of the mL of water samples was adjusted to 6 and the sample passed through the column at flow rate of 6 mL min −1. It uses bicinchoninic acid as the source of copper and turns purple when protein is present.

A spectroscopic reading at nm reveals the amount of protein in the sample.

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The Lowry protein assay uses the phosphate salts of the elements molybdenum and tungsten. metal ions lead, nickel, cadmium, copper and zinc were respectively: , , , and µg L The proposed method The proposed method was applied to measure metal ions in real samples with acceptable accuracy.

Communication UV Spectrophotometric Determination of Cu(II) in Synthetic Mixture and Water Samples Lutfullah,a* Saurabh Sharma,a Nafisur Rahman,a Syed Najmul Hejaz Azmi,b Bashir Iqbal,b MaisaIsmailBilalBaitAmburkb and Zuweina Masoud Hilal Al Barwanib aDepartment of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, India bDepartment of Applied Sciences, Chemistry .

Determination of copper concentrations in water samples using spectrophotometry and bicinchoninic ac
Analytica Chimica Acta (v, #C) |